ii one step cloning kit (Vazyme Biotech Co)
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ii one step cloning kit
Ii One Step Cloning Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 6252 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/step+kit/ClonExpress+II+One+Step+Cloning+Kit/pmc13090716-105-13-18
Average 99 stars, based on 6252 article reviews
Ii One Step Cloning Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 6252 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/step+kit/ClonExpress+II+One+Step+Cloning+Kit/pmc13090716-105-13-18
Average 99 stars, based on 6252 article reviews
ii one step cloning kit - by Bioz Stars,
2026-09
99/100 stars
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Clone Assay:Article Title: Heterogeneous expression of human PNPLA3 triggers algal lipid accumulation and lipid droplet enlargement Article Snippet: Microalgal metabolic engineering holds great promise for algal biofuels.. However, identification of the key lipid metabolic target remains challenging due to its complex regulation.. In this study, we advocated an alternative strategy that potentially rewired lipid metabolism by unprecedented mechanisms. Article Title: Crucial carotenogenic genes elevate hyperaccumulation of both fucoxanthin and β-carotene in Phaeodactylum tricornutum Article Snippet: Burgeoning demand for carotenoids from natural resources has urged to explore the promising metabolic engineering strategy to improve algal systems.. However, existing biological intricacies in algal carotenogenesis warrant identification and exploitation of potential molecular candidate(s) for metabolic engineering to exploit the maximum potential of microalgal cell factories.. Here, we identified two critical carotenogenic genes, including 1-deoxy-D-xylulose 5-phosphate synthase and lycopene cyclase and concurrently overexpressed in Phaeodactylum tricornutum. Article Title: Cystatin B Attenuates Cerebral Ischemia Reperfusion Injury by Inhibiting the JAK2/STAT3 Signaling Pathway. Article Snippet: .. For overexpression, the mouse Cstb coding sequence was amplified with Phanta Max Super- Fidelity DNA Polymerase (P505- d1, Vazyme) and cloned into the pHAGE vector using the ClonExpress II one- Article Title: Occurrence of plastidial triacylglycerol synthesis and the potential regulatory role of AGPAT in the model diatom Phaeodactylum tricornutum Article Snippet: .. The fragment of AGPAT1 was purified by TaKaRa MiniBEST DNA Fragment Purification Kit (Takara, Japan) and the PtAGPAT1 coding sequence was cloned in between the fucoxanthin chlorophyll a/c binding protein (fcp) PfcpC promoter and TfcpA terminator in the expression vector pHY-18 [ ] by ClonExpress II one Expressing:Article Title: Heterogeneous expression of human PNPLA3 triggers algal lipid accumulation and lipid droplet enlargement Article Snippet: Microalgal metabolic engineering holds great promise for algal biofuels.. However, identification of the key lipid metabolic target remains challenging due to its complex regulation.. In this study, we advocated an alternative strategy that potentially rewired lipid metabolism by unprecedented mechanisms. Article Title: Crucial carotenogenic genes elevate hyperaccumulation of both fucoxanthin and β-carotene in Phaeodactylum tricornutum Article Snippet: Burgeoning demand for carotenoids from natural resources has urged to explore the promising metabolic engineering strategy to improve algal systems.. However, existing biological intricacies in algal carotenogenesis warrant identification and exploitation of potential molecular candidate(s) for metabolic engineering to exploit the maximum potential of microalgal cell factories.. Here, we identified two critical carotenogenic genes, including 1-deoxy-D-xylulose 5-phosphate synthase and lycopene cyclase and concurrently overexpressed in Phaeodactylum tricornutum. Article Title: Characterization of the main immunogenic proteins in Brucella infection for their application in diagnosis of brucellosis. Article Snippet: Brucellosis is an important zoonotic bacterial disease widespread in the world.. The key step of control this disease is accurate diagnosis and elimination of diseased animals.. The classic diagnostic methods, such as tube agglutination test, are inaccurate and nonspecific, because of cross-reaction with Yersinia enterocolitica serotype O:9. Article Title: Occurrence of plastidial triacylglycerol synthesis and the potential regulatory role of AGPAT in the model diatom Phaeodactylum tricornutum Article Snippet: .. The fragment of AGPAT1 was purified by TaKaRa MiniBEST DNA Fragment Purification Kit (Takara, Japan) and the PtAGPAT1 coding sequence was cloned in between the fucoxanthin chlorophyll a/c binding protein (fcp) PfcpC promoter and TfcpA terminator in the expression vector pHY-18 [ ] by ClonExpress II one Plasmid Preparation:Article Title: Heterogeneous expression of human PNPLA3 triggers algal lipid accumulation and lipid droplet enlargement Article Snippet: Microalgal metabolic engineering holds great promise for algal biofuels.. However, identification of the key lipid metabolic target remains challenging due to its complex regulation.. In this study, we advocated an alternative strategy that potentially rewired lipid metabolism by unprecedented mechanisms. Article Title: Crucial carotenogenic genes elevate hyperaccumulation of both fucoxanthin and β-carotene in Phaeodactylum tricornutum Article Snippet: Burgeoning demand for carotenoids from natural resources has urged to explore the promising metabolic engineering strategy to improve algal systems.. However, existing biological intricacies in algal carotenogenesis warrant identification and exploitation of potential molecular candidate(s) for metabolic engineering to exploit the maximum potential of microalgal cell factories.. Here, we identified two critical carotenogenic genes, including 1-deoxy-D-xylulose 5-phosphate synthase and lycopene cyclase and concurrently overexpressed in Phaeodactylum tricornutum. Article Title: A rough Brucella mutant induced macrophage death depends on secretion activity of T4SS, but not on cellular Txnip- and Caspase-2-mediated signaling pathway. Article Snippet: Brucella is a facultative intracellular bacterium, dividing into smoothand rough-type Brucella.. Smooth-type Brucella can dissociate into rough mutants with cytotoxicity for macrophages during infection, which is critical for Brucella egress and dissemination.. However, the mechanism of cytotoxicity infected by rough Brucella is incomplete. Article Title: Cystatin B Attenuates Cerebral Ischemia Reperfusion Injury by Inhibiting the JAK2/STAT3 Signaling Pathway. Article Snippet: .. For overexpression, the mouse Cstb coding sequence was amplified with Phanta Max Super- Fidelity DNA Polymerase (P505- d1, Vazyme) and cloned into the pHAGE vector using the ClonExpress II one- Article Title: Occurrence of plastidial triacylglycerol synthesis and the potential regulatory role of AGPAT in the model diatom Phaeodactylum tricornutum Article Snippet: .. The fragment of AGPAT1 was purified by TaKaRa MiniBEST DNA Fragment Purification Kit (Takara, Japan) and the PtAGPAT1 coding sequence was cloned in between the fucoxanthin chlorophyll a/c binding protein (fcp) PfcpC promoter and TfcpA terminator in the expression vector pHY-18 [ ] by ClonExpress II one Homologous Recombination:Article Title: Heterogeneous expression of human PNPLA3 triggers algal lipid accumulation and lipid droplet enlargement Article Snippet: Microalgal metabolic engineering holds great promise for algal biofuels.. However, identification of the key lipid metabolic target remains challenging due to its complex regulation.. In this study, we advocated an alternative strategy that potentially rewired lipid metabolism by unprecedented mechanisms. Purification:Article Title: Crucial carotenogenic genes elevate hyperaccumulation of both fucoxanthin and β-carotene in Phaeodactylum tricornutum Article Snippet: Burgeoning demand for carotenoids from natural resources has urged to explore the promising metabolic engineering strategy to improve algal systems.. However, existing biological intricacies in algal carotenogenesis warrant identification and exploitation of potential molecular candidate(s) for metabolic engineering to exploit the maximum potential of microalgal cell factories.. Here, we identified two critical carotenogenic genes, including 1-deoxy-D-xylulose 5-phosphate synthase and lycopene cyclase and concurrently overexpressed in Phaeodactylum tricornutum. Article Title: Occurrence of plastidial triacylglycerol synthesis and the potential regulatory role of AGPAT in the model diatom Phaeodactylum tricornutum Article Snippet: .. The fragment of AGPAT1 was purified by TaKaRa MiniBEST DNA Fragment Purification Kit (Takara, Japan) and the PtAGPAT1 coding sequence was cloned in between the fucoxanthin chlorophyll a/c binding protein (fcp) PfcpC promoter and TfcpA terminator in the expression vector pHY-18 [ ] by ClonExpress II one Binding Assay:Article Title: Crucial carotenogenic genes elevate hyperaccumulation of both fucoxanthin and β-carotene in Phaeodactylum tricornutum Article Snippet: Burgeoning demand for carotenoids from natural resources has urged to explore the promising metabolic engineering strategy to improve algal systems.. However, existing biological intricacies in algal carotenogenesis warrant identification and exploitation of potential molecular candidate(s) for metabolic engineering to exploit the maximum potential of microalgal cell factories.. Here, we identified two critical carotenogenic genes, including 1-deoxy-D-xylulose 5-phosphate synthase and lycopene cyclase and concurrently overexpressed in Phaeodactylum tricornutum. Article Title: Occurrence of plastidial triacylglycerol synthesis and the potential regulatory role of AGPAT in the model diatom Phaeodactylum tricornutum Article Snippet: .. The fragment of AGPAT1 was purified by TaKaRa MiniBEST DNA Fragment Purification Kit (Takara, Japan) and the PtAGPAT1 coding sequence was cloned in between the fucoxanthin chlorophyll a/c binding protein (fcp) PfcpC promoter and TfcpA terminator in the expression vector pHY-18 [ ] by ClonExpress II one Cloning:Article Title: Characterization of the main immunogenic proteins in Brucella infection for their application in diagnosis of brucellosis. Article Snippet: Brucellosis is an important zoonotic bacterial disease widespread in the world.. The key step of control this disease is accurate diagnosis and elimination of diseased animals.. The classic diagnostic methods, such as tube agglutination test, are inaccurate and nonspecific, because of cross-reaction with Yersinia enterocolitica serotype O:9. Protein Purification:Article Title: Characterization of the main immunogenic proteins in Brucella infection for their application in diagnosis of brucellosis. Article Snippet: Brucellosis is an important zoonotic bacterial disease widespread in the world.. The key step of control this disease is accurate diagnosis and elimination of diseased animals.. The classic diagnostic methods, such as tube agglutination test, are inaccurate and nonspecific, because of cross-reaction with Yersinia enterocolitica serotype O:9. Bacteria:Article Title: Characterization of the main immunogenic proteins in Brucella infection for their application in diagnosis of brucellosis. Article Snippet: Brucellosis is an important zoonotic bacterial disease widespread in the world.. The key step of control this disease is accurate diagnosis and elimination of diseased animals.. The classic diagnostic methods, such as tube agglutination test, are inaccurate and nonspecific, because of cross-reaction with Yersinia enterocolitica serotype O:9. Polymerase Chain Reaction:Article Title: Characterization of the main immunogenic proteins in Brucella infection for their application in diagnosis of brucellosis. Article Snippet: Brucellosis is an important zoonotic bacterial disease widespread in the world.. The key step of control this disease is accurate diagnosis and elimination of diseased animals.. The classic diagnostic methods, such as tube agglutination test, are inaccurate and nonspecific, because of cross-reaction with Yersinia enterocolitica serotype O:9. Article Title: A rough Brucella mutant induced macrophage death depends on secretion activity of T4SS, but not on cellular Txnip- and Caspase-2-mediated signaling pathway. Article Snippet: Brucella is a facultative intracellular bacterium, dividing into smoothand rough-type Brucella.. Smooth-type Brucella can dissociate into rough mutants with cytotoxicity for macrophages during infection, which is critical for Brucella egress and dissemination.. However, the mechanism of cytotoxicity infected by rough Brucella is incomplete. Amplification:Article Title: A rough Brucella mutant induced macrophage death depends on secretion activity of T4SS, but not on cellular Txnip- and Caspase-2-mediated signaling pathway. Article Snippet: Brucella is a facultative intracellular bacterium, dividing into smoothand rough-type Brucella.. Smooth-type Brucella can dissociate into rough mutants with cytotoxicity for macrophages during infection, which is critical for Brucella egress and dissemination.. However, the mechanism of cytotoxicity infected by rough Brucella is incomplete. Article Title: Cystatin B Attenuates Cerebral Ischemia Reperfusion Injury by Inhibiting the JAK2/STAT3 Signaling Pathway. Article Snippet: .. For overexpression, the mouse Cstb coding sequence was amplified with Phanta Max Super- Fidelity DNA Polymerase (P505- d1, Vazyme) and cloned into the pHAGE vector using the ClonExpress II one- Over Expression:Article Title: Cystatin B Attenuates Cerebral Ischemia Reperfusion Injury by Inhibiting the JAK2/STAT3 Signaling Pathway. Article Snippet: .. For overexpression, the mouse Cstb coding sequence was amplified with Phanta Max Super- Fidelity DNA Polymerase (P505- d1, Vazyme) and cloned into the pHAGE vector using the ClonExpress II one- Sequencing:Article Title: Cystatin B Attenuates Cerebral Ischemia Reperfusion Injury by Inhibiting the JAK2/STAT3 Signaling Pathway. Article Snippet: .. For overexpression, the mouse Cstb coding sequence was amplified with Phanta Max Super- Fidelity DNA Polymerase (P505- d1, Vazyme) and cloned into the pHAGE vector using the ClonExpress II one- Article Title: Occurrence of plastidial triacylglycerol synthesis and the potential regulatory role of AGPAT in the model diatom Phaeodactylum tricornutum Article Snippet: .. The fragment of AGPAT1 was purified by TaKaRa MiniBEST DNA Fragment Purification Kit (Takara, Japan) and the PtAGPAT1 coding sequence was cloned in between the fucoxanthin chlorophyll a/c binding protein (fcp) PfcpC promoter and TfcpA terminator in the expression vector pHY-18 [ ] by ClonExpress II one Control:Article Title: Cystatin B Attenuates Cerebral Ischemia Reperfusion Injury by Inhibiting the JAK2/STAT3 Signaling Pathway. Article Snippet: .. For overexpression, the mouse Cstb coding sequence was amplified with Phanta Max Super- Fidelity DNA Polymerase (P505- d1, Vazyme) and cloned into the pHAGE vector using the ClonExpress II one- |
